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Virologica Sinica, 14 (2) : 169-173, 1999
Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C
ChangchunUniversity ofAgricultural andAnimal Sciences,Changchun 130062
Lanzhou Veterinary Institute,Lanzhou 730046
The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies on nucleotide and amino acid sequences were 99.08% and 98.42%, respectively.
  Published online: 5 Jun 1999
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