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Virologica Sinica, 14 (2) : 181-184, 1999
Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells
Tangdu Hospital,The Fourth Military Medical University,Xi'an 710038
The coding region of S genorae segment of Hantaan virus(76/118 strain)was inserted
into the eukarytic expression plasmld pVR1012 The recombinant expression plasmid pVRS22
was c。nstructed.Ver0一E cells were transiently tmnsfected in vitro with pVRS22 plasmid.The
transient expresslon of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indi—
rect immunofluorescence asay(IFA)with monoclonal antibody 5H5 against Hantaan virus
  Published online: 5 Jun 1999
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