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Virologica Sinica, 14 (3) : 217-221, 1999
Review
Overexpression of 28 kDa Protein Encoded by W heat Yellow M osaic Virus RNA2 in E.coli
State Key LaboraZory for Agrobiola-hnology,China Agricultural Oniz~rsizy,Beijing 100094
 Correspondence:
(149.16KB)  
Abstract
With polymerase chain reaction (PCR) a fragment encoding the 28 kDa protein of wheat yellow mosaic virus (WYMV) RNA2 was amplified and constructed into plasmid of pET11a for prokaryotic expression. BL21 (DE3) of \%E.coli\% transformed with the recombinant plasmid of pE2839 was induced to specifically express the 28 kDa protein in high level. The expressed 28 kDa protein was purified from SDS polyacrylamide gels and the antiserum against the protein was raised in rabbit. In Western blotting analysis, the antiserum reacted with the 28?kDa protein.
  Published online: 5 Sep 1999
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