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Virologica Sinica, 14 (3) : 259-264, 1999
Construction of a Recom binant Herpesvirus of Turkey Expressing the Haem agglutinin-Neuram inidase Gene of Newcastle Disease Virus
ColLege ofAnimal Medicine,Nanjing Agricultural University,Nanljlng 210095
A 1.8 kb fragment of Newcastle disease virus (NDV) haemagglutinin neuraminidase (HN) gene was amplified by polymerase chain reaction (PCR) with primers containing enzyme cleavage sites, and inserted into a nonessential gene of herpesvirus of turkey (HVT). Recombinant viruses were screened by Dot blot hybridization with digoxigenin labelled probe and purified by plaque formation technique. The expression of NDV HN gene can be detected by Western blotting in CEF cells infected with recombinant virus. Recombinant HVT strains were replicated stably in cell cultures.
Key Words: NDV;  HN gene;  Recombinant HVT
  Published online: 5 Sep 1999
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