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Virologica Sinica, 14 (4) : 343-352, 1999
Review
Characterization of Proteins and Nucleic Acid of Rice Stripe Virus
Institute of Plant Virology of Fujian Agricultural University,Key Laboratory of Plant Virology of Fujian Province,Fuzhou 350002
 Correspondence:
(272.91KB)  
Abstract
An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some filaments of 13 nm in width and 130-190 nm in length.The basic morphism of RSV particles should be filaments of 3 nm in width and various length.By SDS-PAGE analysis, the molecular weight of disease-specific protein (SP) encoded by vRNA4 was 19. 9 kDa and tha of coat protein (CP) encoded by vcRNA3 was 33. 6 kDa. Whennucleic acid extrcted from the purified RSV was electrophoresed under nondenaturing condition, the she of four dsRNAs(designated as dsRNA1-4 in order of decreasing size) was 4. 9×106, 2. 7×106, 2.0×106 and 1. 7×106 Da, respectively, and that of four ssRNAs (designated as ssRNA1-4 in order of decreasing size) was 3. 0×106, 1. 2×106, 0. 9 ×106 and 0. 8×106 De, respectively. A fifth Segment with a size of 0. 58×10
Key Words:  ;  Rice stripe virus;  Protein;  Nucleic acid;  Serology
  Published online: 5 Dec 1999
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