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Virologica Sinica, 15 (1) : 14-21, 2000
Review
DNA Vaccination of the Induction of Imm une Responses by Hepatitis C Strutural Antigens
Institute of Infectious Disease,The First Affiliated Hospital,Medical hospital of Zhefiang University,Hangzhou 310006,China
 Correspondence:
(215.05KB)  
Abstract

Two recombinant plasmids were constructed. These include the coding regions for the core protein (pC) and for the core, E 1 and E 2 together (pCE 1E 2). These plasmids were transfected into mammalian cells to test their protein expression and injected into the quadriceps muscles of BALB/C mice to measure specific antibodies and cytotoxic T lymphocyte responses. All the recombinant plasmids were shown to express specific antigens transiently and stably in cells and elicited both specific antibody responses and specific cytotoxic T lymphocyte responses. The A value of anticore were 0.358±0.096 (pC) and 0.415±0.127 (pCE 1E 2). The CTL activity of pC was 18.65%±5.71% and 20.07%±11.11% of pCE 1E 2. Genetic immunization can aid the development of hepatitis C virus vaccines by allowing for the rapid construction and evaluation of different expression plasmids as potential immunogens.

  Published online: 5 Mar 2000
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