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Virologica Sinica, 15 (1) : 14-21, 2000
DNA Vaccination of the Induction of Imm une Responses by Hepatitis C Strutural Antigens
Institute of Infectious Disease,The First Affiliated Hospital,Medical hospital of Zhefiang University,Hangzhou 310006,China

Two recombinant plasmids were constructed. These include the coding regions for the core protein (pC) and for the core, E 1 and E 2 together (pCE 1E 2). These plasmids were transfected into mammalian cells to test their protein expression and injected into the quadriceps muscles of BALB/C mice to measure specific antibodies and cytotoxic T lymphocyte responses. All the recombinant plasmids were shown to express specific antigens transiently and stably in cells and elicited both specific antibody responses and specific cytotoxic T lymphocyte responses. The A value of anticore were 0.358±0.096 (pC) and 0.415±0.127 (pCE 1E 2). The CTL activity of pC was 18.65%±5.71% and 20.07%±11.11% of pCE 1E 2. Genetic immunization can aid the development of hepatitis C virus vaccines by allowing for the rapid construction and evaluation of different expression plasmids as potential immunogens.

  Published online: 5 Mar 2000
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