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Virologica Sinica, 15 (1) : 52, 2000
Comparison study about the vp39 gene of Bombyx mori nuclear polyhedrosis virus two isolates from China and Japan
Institute of Virology,Wuhan University
The nuclear capsid protein gene (vp39) of Bombyx mori nuclear polyhedrosis virus (Chinese isolate, BmNPV Ch) was amplified by PCR and inserted into pGEM 3zf(+). The amplified vp39 gene (1 230 bp) was sequenced with silver staining dideoxy chain termination. The coding region is 1 053 bp, and code for 351 amino acids. Comparing with the vp39 gene of BmNPV Ja (Japanese isolate), the homology of the nucleotide and the amino acid sequences is 97.5% and 97.1% respectively. The BmNPV vp39 gene was inserted into the expression vector pRSET A, and transformed into E.coli BL21. This gene from BmNPV Ch is 9 bp longer than that of BmNPV Ja. The insertion of nine nucleotides is found in vp39 gene sequence of BmNPV Ch (CGA at 625 site and GTCGGC at 985-910). Although there are 17 site mutations, only 7 site mutations are replacement mutations. There was no effect to the hydrophilicity and charge of two isolates of VP39. The replacement of anino acids caused by the site mutations made the changes of the seco
  Published online: 5 Mar 2000
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