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Virologica Sinica, 15 (2) : 122-126, 2000
Establishment of Transgenic Mouse Model Carrying HGV Genes
of,Microbiology.SecondMilitary-Medical University,Shanghai 200433,China
The target fragment of HGV genes including C, E1, E2, NS2 and partial NS3 regions was microinjected into the pronuclei of mouse fertilized eggs according to the standard methods. Ten mice containing the HGV genes were identified by the screening of polymerase chain reaction with the primers in NS3 region. The integrated HGV genes were vertically transmitted to the offspring, and the F 1 heterozygous mice from three founders were detected positive. The results of RT PCR indicated that the HGV genes can be transcripted in blood monocytes and hepatocytes. The conventional pathological observation suggested that the transgenic mice had the cloudy swelling, steatosis, hydropic change and inflammatory cellular infiltration. However, serum ATL level showed no significant difference between transgenic and normal mice.
  Published online: 5 Jun 2000
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