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Virologica Sinica, 15 (3) : 226-231, 2000
Studies on the Purification of OPV and its Characterization
Institute of Medical Biology,Chinese Academy of Medical Science&Peking Union of Medical College,Kunming 650107,China
Q Sepharose F.F. was adopted to purify the suspension of oral poliovaccine (OPV), which is prepared from Vero cells. After clarification and concentration by ultrafiltration, the recovery of virus infected titre may attain above 85%. Using column chromatography on Q Sepharose F.F. to purify the concentrated virus suspension, the purified viruses attain 100% recovery of virus infectivity. Dot membrane hybridization was used to detect DNA with the probe of Vero cell genome DNA which was labed with α 32 P dATP, the contents of residual substrate DNA was less than 100 pg/dose. The process of downstream had no significant influence on some biologiccal characters of purified viruses, such as virus morphology, tumorigenicity, rct/40 character and capsid protein. The results indicate that Q Sepharose F.F. is an ideal chromatography material for purifying OPV prepared from Vero cells.
  Published online: 5 Sep 2000
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