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Virologica Sinica, 15 (3) : 234-248, 2000
The Discovery of HBV Core Tissue of Patients with Prom oter M Ⅱtations in Tum our Hepatocellular Carcinoma
Health and Antlepidemic StatHn of Guangxi,Nanning 530021,China
In order to understand the mechanisms of hepatitis B virus (HBV) in the development of hepatocellular carcinoma, the nested PCR was used to amplify HBV core promoter from tumour tissues of 14 HBV DNA positive patients with hepatocellular carcinoma, and 10 serum samples of hepatitis B patients and asymptomatic carriers respectively. The positive samples were sequenced by Sanger method. Ten of the fourteen tumour tissue samples (71.4%) were positive for HBV core promoter. Each sequence of all positive samples had the double mutations at nt 1762(A→T), 1764(G→A) in HBV core promoter and some point mutations around them. The case C14 had more extensive mutations, including deletion of 10 nucleotides. 6 of 10 serum samples of hepatitis B patients were positive for HBV core promoter and 3 of them had the double mutations. four of the ten serum samples of asymptomatic carriers were PCR positive while only 1 of them had the double mutations. These data implicate that the double mutations is most common in HCC patients
  Published online: 5 Sep 2000
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