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Virologica Sinica, 15 (3) : 249-254, 2000
Review
Analysis of Structural Polypeptides and Restriction Patterns of Parocneria orienta Nuclear polyhedrovirus
Wuhan Institute of 
Wirology,Academia Sinica,wuhan 430071
 Correspondence:
(145.77KB)  
Abstract
The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat contained three polypeptides with molecular weight of 31.5 kD, 29.1 kD, 28.6 kD respectively; virion contained 25 structural polypeptides with molecular weight range from 17.6 kD to 114.6 kD. Digestion of PaorNPV DNA with restriction endonucleases, Bam H I, Eco R I, Hin d Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb).
  Published online: 5 Sep 2000
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