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Virologica Sinica, 15 (3) : 272-276, 2000
Direct Detection of Porcine Reproductive and Respiratory Syndrom e Virus of Stillborn Fetus Tissues by Reverse Transcription Polymerase Chain Reaction(RT·PCR)
Animal Husbandryand Veterinary Medical College,Southwest Agricultural University,Chongqing 400716,China
Primers for RT PCR were designed on the basis of the M and N gene sequences of U. S. isolate of PRRSV. The primers have restriction endonuclease ( Eco R I and Bam H I) sites. The tissues of stillborn fetuses of affected swine were detected by RT PCR in 4 pig farms. The samples were RT PCR positive. A gene fragment about 918 bp was amplified by RT PCR, PRRSV were isolated from the tissues of stillborn fetuses. The result showed that PRRSV can be quickly diagnosed by RT PCR, which laid a basis for quick diagnosis of PRRS and further research of the PRRSV isolates of our country.
Key Words: PRRS;  RT PCR;  Detection;  Diagnosis
  Published online: 5 Sep 2000
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