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Virologica Sinica, 15 (3) : 285-290, 2000
Expression of Porcine Growth Horm one Gene in Baculovirus Vector System
Biopharmaceutical Center,State Key Laboratory of Biological Contro1,Zhongshan University,Guangzhou 510275,China
The cDNA sequence encoding porcine growth hormone (pGH) was inserted into the plasmid of pBluescript M13( ). The sequening results showed that a 984 base pair fragment is identical to that reported by Seeburg et al ., with the exception of one different base pair in the ORF. Then, a baculovirus recombinant vector (pX3/4 pGH) with the pGH ORF was constructed and cotransfected Hi 5 cells with the linearized parental virus (AcMNPV OCC -) DNA to produce the recombinant virus AcMNPV pX3/4 pGH OCC +, in which the pGH gene was driven by the synthetic and XIV promoters. The SDS PAGE and Western blot analysis showed that the pGH gene product, 20.7 kDa fusion protein, was obtained. The expressed pGH accumulated up to about 4.48% of the soluble cellular proteins as estimated by CS 930 scanning of the SDS PAGE gel stainning by Coomassie Brilliant Blue R250.
  Published online: 5 Sep 2000
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