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Virologica Sinica, 15 (3) : 308-311, 2000
Expression of Full-length HCV NS3 Gene in E .coli and Its Application in HCV Antibody Detection
Institute of Virology, Wuhan University,Wuhan 430072,China
Full length NS3 gene of a hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural protein gene as template. The amplified fragment (about 1.8 kb) was cloned into plasmid pQE30 and the recombinant plasmid was expressed in JM109. The NS3 protein was purified by NiSO 4 metal chelating resin, and its antigenecity was determined by ELISA, the results showed that the full length NS3 protein was more sensitive than the commercial carboxy terminal domain of NS3 protein in HCV antibody detection.
  Published online: 5 Sep 2000
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