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Virologica Sinica, 15 (4) : 357-360, 2000
Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene
Xuzhou AgriculturatResearch Institute,Xuzhou.Jiangsu,221121 China
A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of GLRaV 3 from America. The expected size of CP gene of GLRaV 3 was amplified by IC (Immuno capture) RT PCR method. The CP gene of GLRaV 3 was cloned into pGEM 3zf(+) vector and sequenced. The nucleotide sequence was compared with American isolate. Result showed that their homology was 94.1%.
Key Words: Grapevine leafroll virus;  GLRaV 3;  IC RT PCR;  CP;  Cl
  Published online: 5 Dec 2000
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