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Virologica Sinica, 15 (4) : 405-408, 2000
Preliminary Study on Chinese Tobacco Leaf Curl Gem nivirus-Guangxi Specie
Institute of Microbiology,Chinese Academy of sciences,Beijing 100080,China
Using DNA of Chinese Tobacco Leaf Curl Virus Guangxi(TbLCV Guangxi)as the template,a 0.4 kb virus specific fragment induding the total common region of the virus and DNA sequence of 52 aa of N terminal of AV1 was obtained by PCR with primers specific to whitefly transmittal gemniviruses,cloned and sequenced.This fragment can basically represent the characteristics of the virus full length genome.The sequence of this fragment is the same as that of chinese tomato yellow leaf curl virus (TYLCV Chi), which indicates that TbLCV Guangxi and TYLCV Chi are the same gemniviruses. According to the sequence of the common region of the 0.4 kb fragment, the virus full length genome DNA was amplified from the infected tobacco plant by PCR with primers back to back.
  Published online: 5 Dec 2000
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