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Virologica Sinica, 15 (4) : 409-413, 2000
Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv
Institute of 
Military Medicine,Nanjing Military Command,PLA,Nanjing 210002,China
The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.
  Published online: 5 Dec 2000
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