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Virologica Sinica, 16 (1) : 15-21, 2001
Research Article
Molecular Cloning and Nucleotide Sequence Analysis of the M Genome Segment of Hantavirus Z37,a Chinese HFRS Vaccine Strain
Zhejiang Provincial Key laboratory of HFRS, Zhejiang Provincial Center Disease Prevention and Control,Hangzhou 310009,China
0ne of the strain of bivalent HFRS vaccine.Z37 strain was isolated from Rattus norvegicus
and identified aS SEO virus by serological test The M segment cDNA of Hantavirus Z37 strain WaS
obtained by reverse transcription and polymerase chain reaction,subsequently cloned into pGEM—T
vector.The sequence of positive recombinants was determined by the method of dideoxy chain term[一
nation,which revea led that the M genomic segment is 3651 nucleotide in length with a predicated bng
open reading frame encoding a protein of 1133 amino acids Comparison with HNT type(76—118.A9,
HV一114 strains)indicatedthattherewere 71 8% ~72.1% homolng y atthe nucleotideleve1.76.2~
76.7% homology at the amino acid leve1.Co mparison with SEO type (R22,L99.80-39 strains)
showed 95.3~ 96 l homology at the nucleotide leve1.95 3~99.2% homology at the amino acid 1ev
d The results of nucleotide and amino acid comparison indicated that Z37 strain is SEO viruses in
molecular Ieve 1.
  Published online: 5 Feb 2001
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