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Virologica Sinica, 16 (1) : 28-33, 2001
Research Article
Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)
1 Natiottal Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050.China
2 Center for Hepatology,Royal Free Campus,University College London,Hamstead.London NW3 2PF,UK
ORF3 and partial ORF1 regions were amplified with RT-PCR from ta~vo patients(T1 and
TII)infected with new genotype of hepatitis E Virus.The PCR products were cloned and sequenced
The results showed that G-C rich region in ORF3 was deleted when amplified with norrflal PCR reac—
tion However.PCR reaction containing G-C melt solution can overcome this problem The sequence
analysis showed that T1 and Tll belong tO a new genotype of HEV which differs from genotype I,II
and III reported.T1 and T11 have 79% -82% .80% -81% and 83% ~85% identical tO genotype I.
II and Ill respectively
  Published online: 5 Feb 2001
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