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Virologica Sinica, 16 (1) : 34-39, 2001
Research Article
Analysis of HIV-1 Sequence W orked Abroad and Subtype in Returned Laborers in Shandong Province
Shandong Provincial Health and Anti—epidemic Station,Jinan 250014,China
 Correspondence:
(195.32KB)  
Abstract
To understand the molecular epidemiologica[characteristic of HIV-1 virus strain in laborers
worked abroad in Shandong province since 2992,11 specimens of peripheral blood of identified HIV
infected eases were sampled and PBM C was separated by Ficoll density gradient centrifuge. The pre—
virus DNA was extracted and the membrane protein gene enzt DNA was multiplied by nested PCR,
and then the sequence of env C2一V3 region*as measured and analyzed.The results indicated that 10
labo rers worked abroad and 1 female—spouse of a labo rer worked abroad weAiTe found catching HIV一1 A
, B ,C,E subtype by gene sequencing and analyzing,with subtype C 7,subtype B 2,subtype A and
E 1 respectively Thus it can be seen that the infection of HIV virus strain in the 1aborers worked
abroad was complex The gene variation rate of these virus strains was greatly different.It is suggest—
ed that we strengthen the detection and control of the 1aborers worked abroad to prevent the spread of
the virus strains from other countries
  Published online: 5 Feb 2001
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