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Virologica Sinica, 16 (1) : 64-67, 2001
Research Article
Study on Purification and Immunogenicity of Rabies Virus Nucleoprotein
Wuhan Institute of Biological Products,Wuhan 430060,China
Abstract:In order tO establish the optimum condition for purification of the nucleopmtein(NP)of rabies virus
by immuno0finity chromatography,the efficient and non-danatulative eluants(Mg-d)Ⅵ忸s obtained by using
ELISA elution model;furthermore.it didn’t damage the activity of NP.Two kind of NPs,expressed by Te—
combimnt vacclnia virus(rVac—N)and~,o3mbinant haculovirus(BRN),were purified by a Sepharose CL 413
column and a 2C12一Sepham~e 4B oolurrm ByWestern-blot and SDS-PAGE.highpurityandgood antigenie,~
intact NPs were identified. purified ribonucleopmtein(RNP) rabies virus 5aG strain was also 0brained.
Aftel"immunized with NP and RNP.mice developed a strong anti-nudeoproteln response and were protected
against a lethal chaHenge of rabies virus CVS strain There were not diference been observed araong the mice
immunized with different purified protein These data indicate that the NPs are antigenical and immunagenical
oomparabletothe authentm rabies RNP aridtherefore present a potantial sour~ ofan dk t ,safeand e∞
nomical subunit vaccine
  Published online: 5 Feb 2001
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