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Virologica Sinica, 16 (1) : 68-73, 2001
Research Article
Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus
1.Dept of Veterinary Medicine, Yangzhou Uniwersity,Yangzhou 225009·China 2 Avian disease and ontology laboratory.USDA.East lansing,MI 48823 USA
The envelope gene of ADOL-4817 strain of avian leukosis v[ros subgroup J(ALV—J)was
amplified by polymerase chain reaction(PCR)and cloned into TA vector.The sequence analysis re—
suits showed that tbe envelope gene is composed of 1 746 bp.1 554 bp of which could be translated in—
to 517 amino acids for gp85 and gp37.The molecular weight of envelope protein is 57.7kD There are
I5 potential glycosy[ation sites in the envelope protein,13 of which is located in gp85,Analysis of se—
quences of envelope gene indicate that ADOL-48 17 showed high degree of sequence identity to other
ALV—J strains,and most closely related to the like—envelope gene of endogenous virus EAV—HP but di—
vergent from these of other ALV subgroup A-E .These data support the hypothesis that envelope gene
of avian leukosis virus suhgroup J maybe acquired hy l:ecombination with expressed sequences
  Published online: 5 Feb 2001
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