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Virologica Sinica, 16 (2) : 155-160, 2001
Research Article
Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect
Institute of Virology,Wuhan University.Wuhan 430072.China
Argyrogramma agnata has been selected as a substitutive host insect for producing Den
drolimus punctatus Cytoplasmic Polyhedmsis Virus(DpCPV),In our experiment.it is very suscepti—
ble to DpCPV.The DpCPV produced in A.agnata is designated Aa-DpCPV The cytoplasmic poly—
hedra body(CPB).the virion size and the shape of Aa—DpCPV are same as that of its original DpCPV
(DpCPV-W 1984) The RNA bands of Aa-DpCPV and DpCPV—W 1984 al1 have 10 RNA segments re—
spectively in 3% PAGE.which molecular weights ranged in size from 2 98×10。to 0 66× 10。Dal—
ton.Aa—DpCPV has the saro.e strong toxicity as that of DpCPV-W 1984(from D punctatus)tO D.
punctatus(Walker)larva So it cai2 he applied tO the pine caterpillar contro1.The DpCPV yield in
A.agnata iS 2.5 x 10 CPB/larva
  Published online: 5 Jun 2001
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