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Virologica Sinica, 16 (2) : 170-174, 2001
Research Article
Maize Dwarf Mosaic Disease Occurred in Hangzhou Isolate Caused by Sugarcane M osaic Virus
1.VirologyLaboratory,ZhejiangAcademy ofAgricultural Sciowes,Hangzhou 310021。China
2 Department of Biological Science and Biotechnology,Zhejiang University。Hangzhou 310027。China
Recently maize dwarf mosaic disease WaS occurred on maize crop seriously in large scale in
Hangzhou district.Purified preparations from the infected maize leaves~ontained numerous filamen—
t0us virus particles of e 750 nm in leng th.Cells of infected plants contained typical pinwheels and lam—
inated aggregates.The coat protein of the virus was 33.6 kD A 1.8 kb fragment of 3’一terminus of
the viral RNA was amplified by RT—PCR,cloned and its sequence was determined。Sequence compar—
isons showed that it shared 71 5% -99 1% homology with isolates of sugarcane mosaic virus.67。8%
~ 68.5% with sorghum mosaic virus and 38。4% ~48.4% with ma ize dwarf mosaic virus,indicating
that the pathogen of this disease on maize in Hangzhou was sugarcane mosaic virus.In addition.the
relationships of sugarcane mosaic virus isolates from different origins all OVer world were discussed
based 0n coat protein sequences
  Published online: 5 Jun 2001
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