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Virologica Sinica, 16 (2) : 175-178, 2001
Research Article
Studies on Apoptosis of Host Cells Induced by Foot-and-M outh Disease Virus
College of Life Sciences,Wuhan University Wuhan 430072,China
Apoptosis of PK—t5 ceils induced by Foot—and-Mouth Disease Virus(FMDV)in vitro was
reported in this paper.Typical cell apoptosis was detected hy ti.se of Hoechst 33258 fluorescence probe.
agarose gel electrophoresis and in situ end-labeling(TUNEL).After PK一15 cells were infected by
titration of 4.8 lg TCIDsD/mL FMDV for 32 h,apoptosis characteristics of nuclear condensation,
fragmentation,accompanied by apoptotic bodies formation(Hoeehst 33258 staining).180—200 inte—
ger-fold sized pieces DNA Ladders(agarose gel e1.ectrophoresis)and strong green fluorescence clots
(TUNEL)were alI exhibited,and cell apoptosis was approximately 20% In addition.the quantira—
tire analysis of apoptosis in PK一15 ceils induced by FMDV showed that apoptosis was correlated with
infection of virus.and it was also tim~dependent.Results indicare that FMDV can induce apoptosis of
host ceils and apoptosis plays an important role in the cytopathogencity effect of FMDV
  Published online: 5 Jun 2001
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