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Virologica Sinica, 16 (2) : 179-182, 2001
Research Article
Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood
Institute of Medical Biology,CAMS & PUMC.Kunming 650118.China
Two pairs of primers were used for amplifying SFV gene with nested—PCR in Rhesus Mon—
key blood The neady 500 bp PCR products were cloned into vector pUC-19.The fragment was de
tected as 465 bp SFV pol gene by sequence analysis.The homology of the fragment to 425 bp pol gene
of the SFV-1 was the highest.it was 92 00% 158 samples of Rhesus Monkey blood were used to de
teet SFV gene with nested—PCR Among them,54 were positive.the poskive rate was 34 2% h ap—
peared a h/gh present of SFV infection in Rhesus M onkey
  Published online: 5 Jun 2001
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