Hello guest, Register  |  Sign In
Virologica Sinica, 16 (3) : 224-228, 2001
Research Article
Cloning and Sequence Analysis of Human Hepatitis B Virus preS Gene Variants from Hangzhou an d Lanzhou in China
1 lnstitute of Biochemistry,Zhejiang University,Hangzhou 310029,China
2.Instituite of Basic Medical Sciences,Peking Uninon Medical College,Chinese Academy of Medical Sciences,Beijing 100005,China
PreS gene fragments were inserted into pBluescript SK after being amplified by PCR from HBV DNA,which was extracted from HBsAg-positive sera(Hangzhou and Lanzhou samples)and the cloned genes were sequenced.The complate nucleotide sequences of the cloned preS genes(preS-HZ and preS-LZ,GenBank accession numbers:Af325674 and AF325675 respectively) were 522bp long,encoding 174aa.The identity of preS-HZ with published preS gene Shagnhai isolate(adr subtype:Gan,et al.,1986),Beijing isolate (adr subtype:Qi,et al.,1989),Japan isolate(adr subtype:Ono,et al.,1983),adw subtype(Valenzuela,et al.,1980),ayw subtype (Galibert,et al.,1979)was respectively 96.7%,96.2%,97.3%,88.7%,84.1% for nucleotide sequence and 96.0%,94.9%,97.1%,85.1%,85.4% for amino acid sequence;correspondingly,preS-LZ was respectively 96.4%,96.2%,96.9%,88.7%,83.7 for nucleotide sequence and 94.9%,94.9%,96.0%,85.1%,84.1% for amino acid sequence.Only two nucleotide substitutions(resulting in two amino acid changes) were observed between preS-HZ and preS-LZ
  Published online: 5 Sep 2001
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved