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Virologica Sinica, 16 (3) : 283-285, 2001
Research Article
Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression
重点开放实验室.上海 200232
DNA fragments of Core(300bp)and NS4(400bp),encoding the nucleocapsid region(Core)protein CS and nonstructural region(NS4)protein NS4a of HCV,have been obtained from HCV genome by PCR,both of the two fragments were liked with C33c(700bp)and formed a chineric gene C33c-Core-NS4(HCV-CCN).The chimeric gene was recombined with expression vector pET24a(+)and was expressed in Escherichia coli.The expressed protein was purified by Ni(+)NTB affinity chromatography.Its molecular weight was about 58kD.Western blotting analysis showed that the chimerical antigen had good antigenicity,which could play an important role in anti-HCV assay.
  Published online: 5 Sep 2001
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