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Virologica Sinica, 16 (3) : 286-288, 2001
Research Article
Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus
1.Institute of virology,Chinese Academy of Sciences,Wuhan 430071,China 2.Sichuan Academy of sciences,Chendu 610081,China
The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by
doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector.Using
the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP,the chifinase gene ofPaorNPV localized OI3_the
fragraents of BamH I-D,EcoR[-A,‰ dⅢ一A,Pst LD and Xho tG/H respectively.Atthe time,the
fragment ofBamH I-D eontniningthe chiA has been cloned.
  Published online: 5 Sep 2001
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