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Virologica Sinica, 16 (3) : 286-288, 2001
Research Article
Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus
1.Institute of virology,Chinese Academy of Sciences,Wuhan 430071,China 2.Sichuan Academy of sciences,Chendu 610081,China
 Correspondence:
(104.61KB)  
Abstract
The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by
doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector.Using
the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP,the chifinase gene ofPaorNPV localized OI3_the
fragraents of BamH I-D,EcoR[-A,‰ dⅢ一A,Pst LD and Xho tG/H respectively.Atthe time,the
fragment ofBamH I-D eontniningthe chiA has been cloned.
  Published online: 5 Sep 2001
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