Hello guest, Register  |  Sign In
Virologica Sinica, 16 (4) : 390-392, 2001
Brief Reports
Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus
慨血mlnsdace o厂V/rdogy,Ch/r~e缸I sc~nce*,Wuhan 430071.0
The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.
  Published online: 5 Dec 2001
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved