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Virologica Sinica, 16 (4) : 390-392, 2001
Brief Reports
Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus
慨血mlnsdace o厂V/rdogy,Ch/r~e缸I sc~nce*,Wuhan 430071.0
 Correspondence:
(92.88KB)  
Abstract
The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.
  Published online: 5 Dec 2001
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