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Virologica Sinica, 17 (1) : 47-50, 2002
Research Article
Construction of HV S and 5’Terminal of S Gene Segment Eukarytic Expression Vectors and Transfection into Vero Cells
Department of infectious Disease, Tangdu Hospital,The 4th.Military Medical University.Xi'an 710038,China
Abstract:In order tO provide some data for study of T cell epitope.HV S and 5’termirmI of S gene
segment were inserted into an eukarytic expression vector pcDNA 3.1/V5一His TOPO.The recombi—
nant expression plasmids peDNA3 1-S and pcDNA3.1-S—N were constructed.Vero cells were trans—
fected in vitro with them.The transient expresion of HV S and 5’terminal of S gene segment in veto
cells w∞ detected by;ndirect immunofluoreseence assay(IFA).
  Published online: 15 Feb 2002
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