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Virologica Sinica, 17 (1) : 66-68, 2002
Research Article
Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus
State Key Laboratory for Biocontrol,Zhongshaa University,Guangzhou 510275,China
Abstract:This research provides the evidence about the identification of haculovirus receptors present
in midgut cell lystes.Rabbit antiserum against purified PDV of SpltM NPV was produced Total midgut
eel1 proteins were prepared with SDS lysis buffer with or wi出OUt 2-mere~,ptoethanal(2-ME).After
SDS—Polyacrylarnid gel eleetmphoresis(SDS—PAGE),the midgut eel1 proteins were tranfered to nitro
cellulose membrance.Transferred proteinS were renaturalized overnight with 4% bovine serum albumin
(BSA).Then with PDV—specific antiserum.a virus overlay protein blot as~y(VOPBA)was performed
to identify the protein that binds PDV of SpltMNPV.h was shown that PDV of SpltMNPV bound c0
three molecules of approximately 40kD,73kDand 85kD in midgut cel1 lysates.
  Published online: 15 Feb 2002
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