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Virologica Sinica, 17 (1) : 73-76, 2002
Research Article
The Isolation and Identification of A New M ember of Tetraviridae Family from China
Institute of Virology,Wuahn University.Wuhan 430072,China
A New member of Tetraviridae family was found and is olate d from Dendrolimus punetatus larvae in China.Purified virion is icosahedral spheroid with the diameter 44nm and 240 identical subunits in the particle with T=4 symmetry.The nucleic acid in the virion was determined to be single-strande d (ss) RNA by means of diphenylamine reaction,orcinol reaction and formaldehyde reaction.The viral RNA showed one band in agarose gel eletrophoresis (5.2kb).Usi n g SDS-PAGE,there were a major polypeptide band of molecular weight 52 kD and a small polypeptide at 39kD. According to the viral taxonomic principle,the virus belongs to Nudaurelia capensis β-like viruses in Tetraviridae.
  Published online: 15 Feb 2002
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