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Virologica Sinica, 17 (1) : 77-81, 2002
Research Article
M odel Establishment of the Cell Persistently Infected by a Field—isolated Virulent Strain of CSFV
1.College of Life Sciences,Wuhan University,Wuhan 430072.China 2.Ch ina Institute of Veterinary Drug Control,Beljing 100081,Ch ina
Abstract:A porcine kidney cell line(PK一15)was infected by a field—isolated virulent strain of classical
swine fever virus(CSFV39) After the infected cells had subeultured 79 passages for near eight
months.a steady modeI of the eell persistently infected by virus has been establisheel,hence the CS—
FV39.PK15 cell line was procured.In this experiment immunofluorescenee,RT-PCR assay and elec—
tron microscopy observation were used to identify the basic viral fearums in the CSFV39一PK15 cel1.
The results showed the 9th,29th.79th CSFV39一PK15 cell pasages still harhored CSFV and they ha d
the radical characteristics of viral persistent infectlon.This endeavor should be the fotmdation for the
further research of CSFV persistent infection.
  Published online: 15 Feb 2002
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