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Virologica Sinica, 17 (1) : 92-95, 2002
Brief Reports
The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product
The Institute of Biochemistry and cell Biology,Chinese Academy Sciences,Shanghai 200031,China
In previous report we have cloned a putative early and late transcriptional gene 1 197 of
prawn baculovirus.In order to detect the biological function of this gene ,1197 gene was inserted into
expressing vector pGEX-3X.The fusion protein expressed with high yield gua$obtained,but it~1)as
foundthattheAsion proteinlocatesininclusion bodies ofE.coli andit causod troubleinitspurfica—
tion.Wefoundthatthe expressed productwithM .of 66kD could bepurifiedfrom inclusion hod—
ies by using a serious of treatments of inclusion bodies r~,ith ug’ea and guanidine hydrochloride and
then by sequential FPLC chromatography.
  Published online: 15 Feb 2002
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