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Virologica Sinica, 17 (2) : 114-118, 2002
Research Article
Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes
College of life sciences.Wuhan University.Wuhan 430072.China
A replication—defective vector(pGPSN1一GFP)base on an infections molecular clone of HFV
(pHSRV13)was constructed by deletion and replacement of the en’u and bet genes with exprssion cas—
setts for neomycin—resistance and EGFP.Vector stocks weir,prodaced by cationic lipid reagents co —
transfection of BHK一21 cells with pGPSNI—GFP and helper plasmid P△ GP These vectors are able to
tra~sfer foreign genes into several vertebrate cells and steadily express them .
  Published online: 5 Apr 2002
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