The Xba I-I fragment located at 18.4~22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.
Correspondence:
(293.35KB)
Abstract
The Xba I-I fragment located at 18.4~22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.