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Virologica Sinica, 17 (2) : 132-136, 2002
Research Article
Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle
Institue of Entomology.Central China Normal University,Wuhan 430079,China
2 Department of Molecular Biology Tongti Hospital.Tongji.Medical College of Huazhong University of Sience and Technology,Wuhan 430030,China
Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes between every two phases were about 6 hours.At 12-18 hours post AcNPV infection,Sf9 cells were arrested in G\-2/M phase.When the cells in G\-1/S phase synchronized by druge were infected by AcNPV,2/3 of the cells was in G\-2/M phase and 1/3 of the cells in S phase.
Key Words: AcNPV;  Cell cycle;  FAGS;  Cell cycle arresting
  Published online: 5 Apr 2002
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