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Virologica Sinica, 17 (2) : 132-136, 2002
Research Article
Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle
Institue of Entomology.Central China Normal University,Wuhan 430079,China
2 Department of Molecular Biology Tongti Hospital.Tongji.Medical College of Huazhong University of Sience and Technology,Wuhan 430030,China
 Correspondence:
(168.67KB)  
Abstract
Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes between every two phases were about 6 hours.At 12-18 hours post AcNPV infection,Sf9 cells were arrested in G\-2/M phase.When the cells in G\-1/S phase synchronized by druge were infected by AcNPV,2/3 of the cells was in G\-2/M phase and 1/3 of the cells in S phase.
Key Words: AcNPV;  Cell cycle;  FAGS;  Cell cycle arresting
  Published online: 5 Apr 2002
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