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Virologica Sinica, 17 (2) : 137-141, 2002
Research Article
Amplification of Dendrolimus puctatus whenshanesis Cytovirus (DpCPV-W)in vitro in Sf21 Cell
Institute of virology,Wuhan University.Wuhan 430072.China
We have studied amphificatlon of DpCPV W in vitro in Sf21 cell ln this paper.The results
showed that DpCPV W could amplify is vitro in Sf2I ce t[and form normal potyhedra Acce~ional TeV
eombinant enhancln in medium coutd synergist the infective rate of DpCPV—W in Sf21 cel1 in vitro.
Bioassay indicated that the toxici’ty of DpCPV—W polyhedra amplified in Sf2 I cell was as violence a5 the
polyhedra amplified in pine caterpillar By using the plaque purfieation technique in Sf21 cell,DpCPV—
W could be purified n a single virion clone Ievd
  Published online: 5 Apr 2002
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