Hello guest, Register  |  Sign In
Virologica Sinica, 17 (2) : 149-152, 2002
Research Article
Cloning,Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain
1.College of Veterinary Medicine,Nanjing Ag iculture University,Nanjing 210095,China
2 Nanjin Entry-Entry lnspection and quranting Beaury Nanjing 210001 China
According to the Genbank open sequence,two pairs of primer were designed in order to am —
plify gE gene and partial gI gene of pseudorabies virus Shanghai strain,respectively.The g£gene and
partial gI gene were obtained by po]ymerase chain reaction(PCR),subsequently cloned into pGEM T
vector.Then both of them were inserted into pUCI8 vector.The recombinant plasmid pgEI deleted
part of gI gene was constructed.
Key Words: Pseudorabies virus;  gl gene;  gene
  Published online: 5 Apr 2002
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved