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Virologica Sinica, 17 (2) : 149-152, 2002
Research Article
Cloning,Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain
1.College of Veterinary Medicine,Nanjing Ag iculture University,Nanjing 210095,China
2 Nanjin Entry-Entry lnspection and quranting Beaury Nanjing 210001 China
 Correspondence:
(143.49KB)  
Abstract
According to the Genbank open sequence,two pairs of primer were designed in order to am —
plify gE gene and partial gI gene of pseudorabies virus Shanghai strain,respectively.The g£gene and
partial gI gene were obtained by po]ymerase chain reaction(PCR),subsequently cloned into pGEM T
vector.Then both of them were inserted into pUCI8 vector.The recombinant plasmid pgEI deleted
part of gI gene was constructed.
Key Words: Pseudorabies virus;  gl gene;  gene
  Published online: 5 Apr 2002
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