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Virologica Sinica, 17 (2) : 153-157, 2002
Research Article
Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus
Lanzhou Veterinary Research Institute of CAAS,Lanzhou 730046,China
 Correspondence:
(178.58KB)  
Abstract
Abstract:Two i.5kb DNA were amplified by reverse transcription and PCR(RT—PCR)from the ep—
ithelial tongue tissue of cattle infected with FMDV akesu/58 strain after 53 passages,and then cloned
A continuous 2,724 nuclco tlde sequence spanning the P3 codiiag region was obtained,including a end—
ing codon TAA 907 amino acids were encoded.3A protein gene was composted of 459 nuclcotides.
encoding 153 amino acids Three 3B(VPg)genes had 69.72 and 72 nuclcotides and encoded 23,24
and 24 amino acids, respectively 639 nuclcotides and 213 amino acids were attributed to 3C and
1,4 13 nucleotides and 471 amino acids to 3D.All proteins were linked by cleavage sites G1u/Gly
(Set).In addition,the varlateCterminal part of 3A protein hasbeenidentified by sequence alignment
with other FMDV strains.
  Published online: 5 Apr 2002
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