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Virologica Sinica, 17 (2) : 185-188, 2002
Research Article
Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs
Kay lab of Aniatat Disease Diagnosis& tmmunotogy,Ministry of Agriculture,Nanjing Agri Univ,Nanjing 210095,China
Abstract:The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho—
mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by
gel filtration chromatogmph with Sepia,arose 4B.The eluates collected were concentrated at 4℃ .The
virus particles in the first part of the eluates could be seen under electron microscopy.The diameter of
virions varified jn 50~ 100nm Sinopotamon denticu缸£“?II artilicia】inlected with the purified virus
became j1].Bv double antibody sandwich EI ISA detection.the results showed that there was signifi—
cai]t diflerence between the jnfected and the centre crabs
  Published online: 5 Apr 2002
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