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Virologica Sinica, 17 (2) : 189-192, 2002
Brief Reports
Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus
Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China
Abstract:PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR.
After digestion by Barn H I and Hin dⅢ.the fragment was cloned into the high level expre~ion vec—
tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E.coli BI 2l(DE3)and ln—
duced by IPTG,high 1evel protein V,’as produced,and the target protein WaS 40% of all proteins.Purifl—
eatlo~ recovery rate was high and up to 92 4% of the target protein before purification.which provides
scientific basis on research of HCM V pathogenesis and diagnosis.
  Published online: 5 Apr 2002
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