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Virologica Sinica, 17 (3) : 195-197, 2002
Research Article
Study on the Endonuelease Site of G1 Region of Hantavirus Genome
Department of Infectious Diseases,Union Hospital,Tong.ji Medical College, Huazhong University of Technology and Science.Wuhan 430022,China
Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of
34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan.The products contained
within G 1 gene of M segm ent, w ere digested with H in C II and Sac I respectively, thereby, han—
tavirus could be typed into HTN and SEO.The results of RFLP—PCR were highly consistent with typ—
ing RT—PCR,and furthermore,RFI P—PCR could quickly screen the variant strain with gene mutation
at the restriction site of HiI"l C II or SacI.
Key Words: Hantavirus;  Virus typing;  RFLP
  Published online: 5 Jun 2002
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