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Virologica Sinica, 17 (3) : 221-225, 2002
Research Article
Sequence Analysis of Com plete L Segm ent of 84FLi, a Hantann Virus Isolated from Xi’an
1.Infectious Diseases Center of P.L.A,Tangdu Ho tal,Fourth Military Medical University,Xi’an 710038,China
2.Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052。Ch ina
 Correspondence:
(185.25KB)  
Abstract
In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i,the
cDNA of L segment was amplified fragment by fragment by RT—PCR .The purified PCR products were
sequenced directly or cloned into pM D18一T vector and then sequenced.The I genom e segm ent is 6533
nucleotides in length with a predicated region(from 38 to 6493)encoding 2151 amino acids.The entire
sequence com position was A 33.39% ,C16.43% ,G20.74% ,T29.44% ,and the GC and AT contents
were 37.17 and 62.83% .Homology analysis showed that the homologies of 84FLi L segment nu—
cleotide sequence with HTN isolates especially Chinese isolates were higher than those of other Han—
tavirus.It showed 83.7% homology with HTN foreign standard strain 76—118。while the amino acid
homology between the two strains was 97.5% .The result suggested that 84FLi strain was one of the
Hantann virus,and highly related tO other Chinese Hantaan Virus iso lates.
  Published online: 5 Jun 2002
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