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Virologica Sinica, 17 (3) : 243-247, 2002
Research Article
Cucum ber M osaic Virus and Tobacco M osaic Virus Proliferation in Transgenic Chili Peppers and its Protoplast
1.Plant protection department of Gausu Agricul tural University,Lanzhou 730070,China
2.Plant Protection Department of NorthzL—estern Agricultural University。Yanglin 712100,China
Transgenic chili pepper plant expressing CM V and TM V coat protein gene were infected
with CM V and TM V to com paring the characteristic of CP—M R in transgenic chili pepper and virus
content in inoculated plants.The results showed that the transgenic chili pepper exhibited high level of
coat protein—mediated resistance to both CM V and TM V .The developm ent of systemic sym ptom was 7
~ 15 days delayed,percentages of plants showing sym ptoms and severity of disease sym ptom in inocu—
lated plants were strongly reduced,and virus accumulat and spread were obviously repressed in inocu—
lated and lewly—developed leaves in CP(+)plants challenged with CMV and TMV.The protoplasts
from transgenic chili pepper expressing CM V—CP and TMV—CP were infected with CMV virion.The
results showed that protoplasts from transgenic chili pepper can also inhibit the virus proliferation.The
protoplasts were infected with 40btg/mL virus concentration,48 hours later,the amount of virus in con—
trol plant protoplasts was 4.2 times than that in CP(+)plant protoplasts.This results demonstrated
that the resistance characteristic of transgenic chili pepper was tO reduce virus proliferation in plants.
  Published online: 5 Jun 2002
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