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Virologica Sinica, 17 (3) : 248-251, 2002
Research Article
The Clone and Expression of the Coat Protein Gene of Potato Virus S in E .coli
Plant Virus Institute of F ian Agriculture and Forestry University,Fuzhou 350008,China
 Correspondence:
(172.23KB)  
Abstract
Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de—
signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR.The gene
was cloned into pGEX一2T vector and then transformed into E .coli DH5a.The gene has high homolo—
gy(95% )comparing with other isolate genes.The expression vector p2TSCP was constructed.The
coat protein gene was expressed in E .coli DH5a and a 58kD protein was analysed by SDS—PAGE.
  Published online: 5 Jun 2002
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