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Virologica Sinica, 17 (3) : 266-269, 2002
Brief Reports
Cloning and Determ ination of S 1 Gene of IBV GD05 Strain and Its Expression in E .coli.
1.Biopharmaceutic R&D center of Jinan University,Guangzhou 510632,China
2.Department of veterinary medicine South china Agriculture University,@uangzhou 510642,China
3.Depa rtment of Sericulture, South china Ag riculture University,Guangzhou 510642,China
Acording tO Avian Infectious Brochitis virus(IBV)Beaudette strain S 1 gene sequence,a
pair of primers were designed and synthesized.W ith the primers, IBV Guangdong isolation strain
GD05 S 1 gene was successively amplified by RT — PCR .PCR product was digested w ith BstY I,Hae
III and Pst I respectively,the result showed RFLP pattern of was the same as that of M41 S 1 gene.
IBV GD05 strain was thought as M ass serotype primaryly.IBV GD05 S 1 gene was cloned into pGEM
— T vector and sequenced, its sequence was consisted of 1611 base pairs.By comparison , the nu—
cleotide sequence was 97.14% identical tO that of IBV M 41. IBV GD05 S 1 gene was subcloned into
expression vector pET21 d.SDS—PAGE experiment showed that it expresed in E .coli.
  Published online: 5 Jun 2002
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