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Virologica Sinica, 17 (4) : 304-307, 2002
Research Article
Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation
1.Hubei Medical stuff College,Jingzhou 434000,China
2.Medical College,Wuhan University,Wuhan 430071,China
Using molecular cloning technique,HPV16 E7 gene was cloned into eukarytic expression
vector.HPV16 E7 DNA vaccine was constructed and Balb/c mice were immunized by intradermal ad—
ministration with HPV16 E7 vaccine.After immunization,mice spleen lymphocytes were prepared and
restimulated by E7 protein in vitro.Specific lympproliferation were detected by MTT colorimetric as—
say.Because detection of specific lymphoproliferation is a simple and efficient way,which can reflect
cell—mediated immunity.The study showed that the construction of HPV16 E7 vaccine was correct.It
can induce a specific cell—mediated immunization
  Published online: 5 Nov 2002
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