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Virologica Sinica, 17 (4) : 326-330, 2002
Research Article
Expression of Full Length and N Termial Truncated Enhancin from Trochoplusia ni granulovirus in AcM NPV and Analysis of Their Activit
Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University,Shanghai 200433,China
Enhancin is a group of baculovirus proteins capable of increasing the infectivity of viruses in
insect larvae,as well as the insecticidal effect of other biology control agents. In order to study the
structure and function of enhancin from Trichoplusia i gran ulovirus, recombinant baculoviruses
were constrcuted to express truncated forms of enhancin with 150, 186 and 250 am ino acids deleted
from N terminal of native enhancin,respectively.The truncated enhancins were expressed successfully
in Tn一5B1—4 cells. In vitro peritrophic membrane assay showed that all three truncated enhancin lost
their mucin degrading ability,while a full length recombinant enhancin was active in the assay.It is
concluded that the N terminal amino acids is essential for the mucin degrading activity of enhancin.
  Published online: 5 Nov 2002
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